The Primary Structure of Escherichia coli K12 2-Deoxyribose 5-Phosphate Aldolase
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چکیده
منابع مشابه
Deoxyribose Phosphate Aldolase from Rat Liver.
The activity and properties of deoxyribose phosphate aldolase from Escherichia coli and Lactobacillus plantarum have been described in detail by Racker (1) and Pricer and Horecker (2). The enzyme from E. coli was shown to be active at pH values between 6.0 and 8.0 in tris(hydroxymethyl)aminomethane and phosphate buffers without exhibiting a sharp optimum (1). The enzyme from L. plunturum was sh...
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DERA, 2-deoxyribose-5-phosphate aldolase, catalyzes the retro-aldol cleavage of 2-deoxy-ribose-5phosphate (dR5P) into glyceraldehyde-3-phosphate (G3P) and acetaldehyde in a branch of the pentose phosphate pathway. In addition to the physiological reaction, DERA also catalyzes the reverse addition reaction and, hence, is an interesting candidate for bio-catalysis of carbo-ligation reactions, whi...
متن کاملAcrolein, an irreversible active-site-directed inhibitor of deoxyribose 5-phosphate aldolase?
The enzyme deoxyribose 5-phosphate aldolase was irreversibly inactivated by the substrate analogue acrolein with a pseudo-first-order rate constant of 0.324 min-1 and a Ki (apparent) of 2.7 x 10(-4) m. No inactivation was observed after prolonged incubation with the epoxide analogues glycidol phosphate and glycidaldehyde. It is suggested that the acrolein is first activated by forming a Schiff ...
متن کاملNucleotide sequence of the purM gene encoding 5'-phosphoribosyl-5-aminoimidazole synthetase of Escherichia coli K12.
5'-Phosphoribosyl-5-aminoimidazole synthetase (EC 6.3.3.1), encoded by the purM gene of Escherichia coli, catalyzes the synthesis of 5'-phosphoribosyl-5-aminoimidazole from 5'-phosphoribosylformylglycinamidine. The purM gene was subcloned from the Clarke and Carbon (Clarke, L., and Carbon, J. (1976) Cell 9, 91-99) plasmid pLC1-41 and the nucleotide sequence determined. The mature protein, as de...
متن کاملDeoxyribose Aldolase from Lactobacihs plantarum
The fermentation of deoxyribose in Escherichia coli (1, 2) and in Lactobacillus plantarum (3, 4) appears to involve 2-deoxyribose 5-phosphate as an intermediate. Racker (2) has described an enzyme, deoxyribose phosphate aldolase, which catal.yzes the reversible cleavage of deoxyribose 5-phosphate to acetaldehyde and glyceraldehyde 3-phosphate. The enzyme occurs in E. coli and in animal tissues ...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1982
ISSN: 0014-2956
DOI: 10.1111/j.1432-1033.1982.tb06719.x